Mikroskoob-ka fluorescence waa nooc ka mid ah mikroskoob indhaha ah kaas oo isticmaala il iftiin xoog leh si uu u iftiimiyo muunada iyo kicinta fluorochromes ee muunada. Iftiinka muunada waxaa badanaa lagu sameeyaa il iftiin ah oo siida iftiinka ultraviolet. Waxaa si weyn loogu isticmaalaa dhinacyada noolaha, caafimaadka iyo warshadaha.
Sidee buu u shaqeeyaa mikroskoob-ka fluorescence?
Mikroskoob-ka fluorescence wuxuu isticmaalaa meerkuri ama nalka xenon si uu u soo saaro iftiinka ultraviolet. Iftiinku wuxuu soo galayaa mikroskoob wuxuuna ku dhuftaa muraayadda dichroic - muraayad ka tarjumaysa hal mawjadaha dhererka oo u oggolaanaya kala duwanaansho kale inuu dhex maro. Muraayada dichroic waxay ka tarjumaysaa iftiinka ultraviolet ilaa muunada. Muunadaha qaarkood waxay si dabiici ah ugu soo baxaan iftiinka ultraviolet sababtoo ah waxay ka kooban yihiin walxo fluorescent ah sida chlorophyll. Haddii muunada la daawado aysan si dabiici ah u iftiimin, waxaa lagu shubi karaa midabyo fluorescent ah oo loo yaqaan fluorochromes.
Muxuu ka kooban yahay mikroskoob-fuloorescence?
1. Isha Iftiinka
Ilaha iftiinka ugu caansan waa meerkuri, xenon, iyo LEDs. Meerkurigu wuxuu bixiyaa tayada ugu fiican ee iftiinka mikroskoobyada fluorescence. Nalalka LED-yadu aad ayay caan u noqonayaan sababtoo ah way ka kharash yar yihiin ilaha kale waxayna cunaan koronto yar.
| Meerkuriga nalka | Xenon Lamp | LED | |
| Dhererka Mawjadaha Iftiinka Lasoosaaray | 350-370 nm | 400nm ilaa 450nm | 400-700 nm |
| Cimriga (saac) | 200-300 | 400-600 | 10000 |
| Faa'iidada | 1. Iftiinka Xoogga Sare, Ilaha Iftiinka ugu Badan. 2. Waxay sii daysaa ultraviolet xoog leh iyo buluug-violet si ay u kiciso dhammaan noocyada fluorophores. 3. Sawirro dhalaalaya oo midab leh. | 1. xoogga muuqaalku waa mid deggan. 2. Xoog xoog leh oo xoog leh oo ku jira infrared iyo dhexda dhexe. | 1. Si fudud loo beddeli karo. 2. Looma baahna in la kululeeyo. 3. Waa la dami karaa oo dami karaa isla markiiba. 4. Xakamee xoogga isha iftiinka. |
| Khasaaro | 1. Cimri gaaban. 2. Waqti dheer oo kululaynta. | 1. Sanduuqa korantada DC ee danab yar oo gaar ah ayaa looga baahan yahay kulaylka xad dhaafka ah. 2. Ka Qaalisan Lambabka Mercury | 1. Iftiinka UV wuu ka daciifsan yahay nalka meerkuriga. 2.Wavelength kasta oo iftiin ah wuxuu u baahan yahay LED gaar ah sababtoo ah xajmiga cidhiidhiga ah ee ay sii daayaan. |
Ku-xidhka Dheelaha Mercury
Lifaaqa LED-ka Fluorescent
2. Shaandheynta xiisaha
Shaandheeyaha kicinta ayaa lama huraan u ah hawlgalka mikroskoob ee fluorescence. Waxay dhaaftaa iftiinka hirarka dhererka gaaban, kaas oo dheeha fluorescent uu nuugi karo. Sidoo kale, waxay xannibaysaa ilaha kale ee iftiinka xiisaha leh.
Shaandhaynta xiisaha (B,G,U,V)
3. Muraayada Dichroic
Muraayada dichroic waa nooc ka mid ah shaandhada indhaha oo ka tarjumaysa iftiinka mawjadaha dhererka qaarkood iyadoo gudbinaysa kuwa kale. Waxa loo adeegsadaa mikroskoobyada fluorescence si loo kala saaro xamaasadda iyo hirarka qiiqa.
4. Shaandheynta sii daynta
Shaandheeyaha qiiqa wuxuu dhaafaa kaliya dhererka hirarka uu sii daayo fluorophore wuxuuna xannibaa dhammaan iftiinka aan loo baahnayn ee ka baxsan band-gan - gaar ahaan hirarka kacsanaanta.
5. Midabyada fluorescent
Midabada fluorescent waa isku-dhisyo dabiici ah oo leh hanti dhalaalaysa, taas oo ay ku samayn karaan sawir fluorescent ah iyagoo soo saaraya iftiin cagaaran oo muuqda ka dib markii ay ku faraxsan yihiin iftiinka ultraviolet ee aadka u iftiimaya. Midabaynta fluorescent ee inta badan la isticmaalo waa; DAPI (49,6-diamidino-2-phenylindole), orange acridine, auramine-rhodamine, Alexa Fluors, ama DyLight 488.
Immisa nooc oo mikroskoobyada fluorescence ah?
1. Mikroskoob ee Epifluorescence toosan:
Waa nooca ugu badan ee mikroskoob ee fluorescence. Dareenka fluorophore-ka iyo ogaanshaha fluorescence-ka waxa lagu sameeyaa dariiq iftiin la mid ah (sida ujeeddada). Inta badan mikroskoobyada fluorescence, gaar ahaan kuwa loo isticmaalo cilmiga nolosha, ayaa ah naqshadda epifluorescence.
BS-2081F Cilmi Baadhista Mikroskoob ee Biyolojiyeed ee Fluorescent
2. Microscope Confocal Fluorescence:
Confocal Fluorescence Microscope: Noocan ah mikroskoob ee fluorescence wuxuu isku daraa iskaanka laysarka iyo iftiinka fluorescent si loo soo saaro sawir. Waxaa loo isticmaali karaa codsiyo kala duwan, sida barashada unugyada iyo unugyada, ogaanshaha borotiinka iyo walxaha kale ee unugyada, iyo cabbiraadda dhumucda alaabta.
BS-2081F Cilmi Baadhista Mikroskoob ee Biyolojiyeed ee Fluorescent
3. Mikroskoobkii Fluorescence-ga rogan
Isha iftiinka mikroskoob ee noocaan ah iyo kondhiyaha ayaa ku yaal xagga sare, una jeedda hoos. Xagasha iftiiminta waa in ay ahaataa 90 darajo marka loo eego dusha muunada la baarayo.
BS-2095F Mikroskoob ee Fluorescent Rogmaday
Waa maxay faa'iidooyinka iyo khasaaraha ee mikroskoobyada fluorescence?
Faa'iidooyinka:
-Waxay suurtagelisaa fiirsashada unugyada noolaha iyada oo aan waxyeello u geysan
- Waxay ku siisaa sawirro xallin sare leh midabyo sax ah
- Waxay ogolaataa daraasadda hababka nool ee unugyada
Loo isticmaalo in lagu aqoonsado noocyo kala duwan oo unugyo ah oo ku jira unugyada, sida borotiinnada ama asiidhka nucleic-ka.
Khasaaraha:
-Waxay ogolaataa oo kaliya in la eego qaab-dhismeedyo gaar ah oo gudaha unug ah oo lagu sumadeeyay dheeha fluorescent.
- Sawir-qaadista ay sabab u tahay kicinta elektarooniga ah inta lagu guda jiro habka fluorescence waxay saameyn kartaa molecules fal-celinta ee dheeha fluorescent. Natiijo ahaan, midabada falceliska ahi waxay lumin karaan hantidooda kiimikaad ee xoojinta qiiqa qiiqa.
Unugyadu waxay u nugul yihiin saamaynta sawir-qaadista ka dib markii ay ku dheehdaan dheeha fluorescent, sida molecules fluorophore-ku waxay nuugaan tamarta sare ee photons ee iftiinka dhererka gaaban.
Waa maxay codsiyada mikroskoob ee fluorescence?
Mikroskoobyada Fluorescence ayaa si weyn loogu isticmaalaa dhinacyo kala duwan oo cilmi baaris iyo codsi ah oo ay ku jiraan biochemistry, bayoolaji unugyada, microbiology, immunology, iyo daawada.
1. Dhinaca cilmiga bayoolajiga, mikroskoobku fluorescence wuxuu awood u siinaya aqoonsiga saxda ah ee faahfaahsan ee unugyada gacanta iyo qaybaha unugyada hoose iyo hawlaha iyada oo la kaashanayo calaamadaynta dheeha fluorescent.
2. Dhinaca caafimaadka, mikroskoobku fluorescence wuxuu isticmaali karaa reagents fluorescent si loo ogaado joogitaanka iyo qaybinta bakteeriyada iyo fayrasyada, ama si ay u caawiyaan calaamadaynta bartilmaameedyada qalliinka si loo fududeeyo qalliinka.
3. Dhinaca macdanta, mikroskoob-ka fluorescence waxaa inta badan loo isticmaalaa in lagu barto walxaha leh sifooyinka ifay-dhaleedka iskiis ah, sida asphalt, petroleum, dhuxusha, graphene oxide iyo macdano kale.
4. Sayniska agabka, mikroskoob-ka fluorescence waxaa loo isticmaali karaa warshadaha dunta ama warshadaha waraaqaha si loo falanqeeyo agabka ku salaysan fiber-ka.
Waqtiga boostada: Mar-14-2023