Mikroskoobyada Fluorescence waxa ay beddeshay awooddayada aan ku aragno oo aan u baranno shaybaarrada noolaha, taas oo noo oggolaanaysa in aan dhex galno adduunka qallafsan ee unugyada iyo molecules. Qayb muhiim ah oo ka mid ah microscopy fluorescence waa isha iftiinka ee loo isticmaalo in lagu kiciyo molecules fluorescent ee muunada dhexdeeda. Sanadihii la soo dhaafay, ilo iftiin kala duwan ayaa la shaqaaleysiiyay, mid walbana wuxuu leeyahay astaamo iyo faa'iidooyin gaar ah.
1. Mercury Lamp
Nalalka meerkuriyada ee cadaadiska sarreeya, oo u dhexeeya 50 ilaa 200 watts, ayaa lagu dhisay iyadoo la isticmaalayo muraayadda quartz waana qaab wareegsan. Waxay ka kooban tahay xaddi meerkuri ah oo gudaha ah. Marka ay shaqeyso, dheecaanku wuxuu ka yimaadaa inta u dhaxaysa laba electrodes, taasoo keenaysa meerkuri inuu uumi baxo, cadaadiska gudaha ee wareegga ayaa si degdeg ah u kordha. Habkani wuxuu caadi ahaan qaataa ilaa 5 ilaa 15 daqiiqo.
Sii daynta nalka meerkurigu-cadaadiska sareeyo waxa uu ka dhashaa kala daadsanaanta iyo dhimista molecules mercury inta lagu jiro dareeraha korantada, taasoo keenta soo daynta photons-ka iftiinka.
Waxay soo saartaa ultraviolet xoog leh iyo iftiin buluug-violet ah, taas oo ka dhigaysa mid ku habboon waxyaabo xiiso leh oo kala duwan oo fluorescent ah, taas oo ah sababta loo isticmaalo microscopy fluorescence.
2. Laambadihii xenon
Ilaha kale ee caadiga ah ee loo isticmaalo iftiinka cad ee mikroskoobyada fluorescence waa nalka xenon. Laambadaha xenon, sida laambadaha meerkuriga, waxay bixiyaan hirar ballaadhan oo dhererkoodu yahay ultraviolet ilaa infrared-ku dhow. Si kastaba ha ahaatee, waxay ku kala duwan yihiin spectra excitation.
Laambadaha Mercury waxay xooga saarayaan sii dayntooda meelaha u dhow-ultraviolet, buluug, iyo cagaar, taas oo hubisa jiilka calaamadaha fluorescent ee dhalaalaya laakiin la socda sawir-toosiye xooggan. Sidaa darteed, nalalka HBO waxaa caadi ahaan loogu talagalay muunado go'an ama sawir-qaadista iftiinka daciifka ah. Taas bedelkeeda, ilaha nalka xenon waxay leeyihiin muuqaal kicinta jilicsan, taas oo u oggolaanaysa isbarbardhigga xoojinta hirarka kala duwan. Dabeecaddani waxay faa'iido u leedahay codsiyada sida cabbiraadaha xoojinta ion calcium. Laambadihii xenon waxa kale oo ay soo bandhigaan xamaasad xoog leh oo ku saabsan qiyaasta infrared-ka dhow, gaar ahaan agagaarka 800-1000 nm.
Laambadaha XBO waxay leeyihiin faa'iidooyinka soo socda marka loo eego nalalka HBO:
① Xoog badan oo lebis ah
② Xoogan muuqaal xoog leh oo ka jira gobollada infrared iyo kuwa dhexe
③ Wax soo saar tamar oo ka weyn, taasoo sahlaysa in la gaadho daloonka ujeeddada.
3. LEDs
Sanadihii la soo dhaafay, tartan cusub ayaa ka soo baxay saaxadda ilaha iftiinka florescence microscopy: LEDs. Nalalka LED-yadu waxay bixiyaan faa'iidada daminta degdega ah ee millise seconds, yaraynta muunada wakhtiyada soo-gaadhista iyo kordhinta cimriga shaybaarrada jilicsan. Intaa waxaa dheer, iftiinka LED wuxuu soo bandhigaa suus degdeg ah oo sax ah, isagoo si weyn u yareynaya sawir-qaadista inta lagu jiro tijaabooyinka unugyada muddada-dheer.
Marka la barbardhigo ilaha iftiinka cad, LED-yadu waxay caadi ahaan ku sii daayaan xajmi yar oo kacsan. Si kastaba ha ahaatee, kooxo badan oo LED ah ayaa la heli karaa, taas oo u oggolaanaysa codsiyada fluorescence midabyo badan oo kala duwan, samaynta LED-yada doorasho caan ah oo caan ah oo casriga ah ee muraayadaha casriga ah.
4. Isha Laysarka
Ilaha iftiinka laser-ka ayaa ah mid monochromatic ah iyo jihayn, taas oo ka dhigaysa inay ku habboon yihiin mikroskoobyada xallinta sare, oo ay ku jiraan farsamooyinka xallinta sare sida STED (Stimulated Emission Depletion) iyo PALM (Photoactivated Localization Microscope). Iftiinka Laser-ka waxaa sida caadiga ah loo doortaa si uu u dhigmo dhererka hirarka xiisaha gaarka ah ee looga baahan yahay fluorophore-ka bartilmaameedka ah, isagoo siinaya xulasho sare iyo saxnaanta kicinta fluorescence.
Doorashada isha iftiinka mikroskoob ee fluorescence waxay ku xiran tahay shuruudaha tijaabada ee gaarka ah iyo sifooyinka muunada. Fadlan xor u noqo inaad nala soo xidhiidho haddii aad u baahan tahay wax gargaar ah
Waqtiga boostada: Seb-13-2023